The Stories that Fluorescent Signals from Whole Cells can Reveal
New fluorescent compounds are important chemo-enzymatic tools to reveal enzymatic activities in vitro and in vivo environments. These applications can reveal organelles, membranes, nucleus and enzymatic activities with fluorogenic probes, signaling the presence of specific enzymes. In this context, several fluorogenic compounds were synthesized, with novel photo physical characterizations and specific biological applications are discussed. As the simultaneous detection of hydrolases in microorganisms, with probes that present different fluorophores (resorufin and umbelliferone) and functional groups (epoxide and ester) that, after enzymatic action, culminate in the same chemical cascade of fluorophore release. This methodology is called multiplex screening. Revealed the enantiomeric excesses (ee) and conversions of enzymatic reactions, using enantiomerically enriched umbelliferone derivatives probes with a methodology implemented by our research group, named Quick-ee. Also in the biological context, the elucidation of an enzymatic cascade present in breast and prostate human tumor cells, involving PSTP (serine/threonine phosphatases) and ADH (alcohol dehydrogenases), important enzymes associated with tumor activity. Finally a novel fluorescent substance with a potential photodynamic therapy in prostate cancer cell will be discussed.