Phytochemical and Biological Studies on the Selected Medicinal Plants of Dhading and Makwanpur Districts of Nepal and Isolation of Chemical Constituemnts from Myrica Esculenta
Phytochemical and biological screenings of some medicinal plants collected from Dhading and Makwanpur District were performed. Phytochemical screening suggest that the selected plant sample contains Alkaloids, Flavonoids, Terpenoids, Glycosides, Quinones, Reducing sugars, Polyphenols and Saponins. Methanolic extracts of bark of Myrica esculenta showed high toxicity against brine shrimp naupili having LC50 value 20.89μg/ml. Among the ten selected medicinal plants, total phenolic content and total flavonoid content were also found highest in bark of Myrica esculenta having the values 262±6.64 mg/g gallic acid equivalent and 151.23±4.63 mg/g quercetin equivalent respectively. The antioxidant properties of ten samples were evaluated by using DPPH assay and their IC50 values were calculated. IC50 value of M. esculenta was found as the lowest (46.81 µg/ml) and closest to that of Ascorbic acid (41.34 µg/ml) taken as standard. Two pure compounds IPC1 and IPC2 were isolated from methanolic extract of bark Myrica esculenta by column chromatography. The compound IPC1 was proposed as β-sitosterol with the aid of Co-TLC and melting point of authentic sample. The compound IPC2 is under process for its structure elucidation.